For months - months! - I have been laboring over this problem at work. I have two mice, each with a different genotype. PCR done by my coworkers shows one mouse has a transgene, the other does not. However, I keep getting results over and over again that say that both these mice have the transgene. So either the tests I'm doing are wrong, or something's going on with these mice. I assumed it was my tests that were failing somehow, and I felt stupid and like a really bad scientist.
Today I learned that the both mice have the transgene, and that our PCR method of genotyping gives false positive results. So, there's no way I could have gotten the results I was hoping for. It is these sorts of time-wasting experiences I sincerely hope will not find their way to the forefront of my mind as I lay on my deathbed...
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